Association of circulating CEACAM1 levels and insulin sensitivity in gestational diabetes mellitus.

The aim of this study was to estimate the levels of circulating carcinoembryonic antigen-related cell adhesion molecule 1 (CEACAM1) in subjects with gestational diabetes mellitus (GDM) and investigate the relationships between CEACAM1 and GDM. Circulating CEACAM1 levels were measured by ELISA kit in 70 women with GDM and 70 normal glucose tolerance (NGT) pregnant women. Blood samples were collected to detect fasting plasma glucose (FPG), fasting insulin (FINS) and glycosylated hemoglobin (HbA1c) levels in all participants. Insulin sensitivity index (ISOGTT) was calculated to assess insulin sensitivity.

Correlation analysis was performed between serum CEACAM1 levels and other parameters.Circulating CEACAM1 levels were higher in the GDM group than that in the NGT pregnant group, however, the difference showed no statistical significance (1889.82 ± 616.14 vs 1758.92 ± 433.15 pg/ml, p > 0.05). In GDM group, CEACAM1 was positively correlated with ISOGTT (R = 0.39, P = 0.001), while negatively with 1 h post-meal plasma insulin level (1hPINS) (R = -0.32, P = 0.008), 2 h post-meal plasma insulin level (2hPINS) (R = -0.33, P = 0.006) and area under curve of insulin (AUCI) (R = -0.36, P = 0.002) when adjusting for maternal age and gestational age.

The present study showed that circulating CEACAM1 levels did not differ in both GDM and NGT groups. However, we found a significant positively correlation between CEACAM1 and insulin sensitivity in the GDM group.

DACH1, a novel target of miR-218, participates in the regulation of cell viability, apoptosis, inflammatory response, and epithelial-mesenchymal transition process in renal tubule cells treated by high-glucose.

Objective: This report was designed to assess the functional role of miR-218/dachshund family transcription factor 1 (DACH1) in diabetic kidney disease (DKD) and investigate its possible molecular mechanism.Materials and Methods: From the GEO database, we downloaded different datasets for analyzing the expression of miR-218 and DACH1 in DKD. TargetScan was adopted to predict the binding sites between miR-218 and DACH1, which was further verified by dual-luciferase reporter assays. The renal proximal tubule cells (HK-2) treated with high glucose (HG) were used as an in vitro model.

QRT-PCR and western blot were used to determine the expression of DACH1 and other relative factors. Cell counting kit-8 and flow cytometer were applied to detect cell viability and apoptosis. The levels of inflammatory cytokines were determined by an ELISA assay.

NKG2D antibody (PE)
MBS834175-01mg	MyBiosource	0.1mg	EUR 700

NKG2D antibody (PE)
MBS834175-5x01mg	MyBiosource	5x0.1mg	EUR 3010

Human NKG2D Antibody
36795-05111	AssayPro	150 ug	EUR 215

CD314 (NKG2D) Antibody
abx201090-01mg	Abbexa	0.1 mg	EUR 477.6

NKG2D antibody (biotin)
61R-1605	Fitzgerald	100 ug	EUR 458
Description: Armenian Hamster monoclonal NKG2D antibody (biotin)

NKG2D antibody (biotin)
MBS834983-01mg	MyBiosource	0.1mg	EUR 765

NKG2D antibody (biotin)
MBS834983-5x01mg	MyBiosource	5x0.1mg	EUR 3285

NKG2D antibody (allophycocyanin)
61R-1778	Fitzgerald	100 ug	EUR 376
Description: Rat monoclonal NKG2D antibody (allophycocyanin)

NKG2D antibody (allophycocyanin)
MBS833721-005mg	MyBiosource	0.05mg	EUR 640

NKG2D antibody (allophycocyanin)
MBS833721-01mg	MyBiosource	0.1mg	EUR 1030

NKG2D antibody (allophycocyanin)
MBS833721-5x01mg	MyBiosource	5x0.1mg	EUR 4475

CD314 (NKG2D) Antibody (PE)
abx201092-100tests	Abbexa	100 tests	EUR 927.6

CD314 (NKG2D) Antibody (APC)
abx201093-100tests	Abbexa	100 tests	EUR 828

CD314 (NKG2D) Antibody (FITC)
abx201091-100tests	Abbexa	100 tests	EUR 560.4

Anti-Mouse NKG2D Antibody
103-M441	ReliaTech	100 µg	EUR 399
Description: NKG2D is a type II transmembrane protein with an extracellular C-type lectin domain. It occurs as a disulfide-linked homodimer that associates with either the DAP12 or DAP10 transmembrane adapter proteins. NKG2D is expressed on NK cells and some T cell subsets, where it functions as an activating receptor to trigger cytolytic activity and cytokine secretion. Ligands for human NKG2D include MICA, MICB, and ULBP-1, -2, -3, and -4. Mouse NKG2D ligands include H60, MULT-1, and Rae-1 alpha, beta, gamma, delta, and epsilon.

Anti-Human NKG2D Antibody
101-M585	ReliaTech	100 µg	EUR 399
Description: NKG2D is a type II transmembrane protein with an extracellular C-type lectin domain. It occurs as a disulfide-linked homodimer that associates with either the DAP12 or DAP10 transmembrane adapter proteins. NKG2D is expressed on NK cells and some T cell subsets, where it functions as an activating receptor to trigger cytolytic activity and cytokine secretion. Ligands for human NKG2D include MICA, MICB, and ULBP-1, -2, -3, and -4. Mouse NKG2D ligands include H60, MULT-1, and Rae-1 alpha, beta, gamma, delta, and epsilon.

CD314 (NKG2D) Antibody (Biotin)
abx201094-01mg	Abbexa	0.1 mg	EUR 460.8

NKG2D Antibody HRP Conjugated
MBS9460103-01mL	MyBiosource	0.1mL	EUR 595

NKG2D Antibody HRP Conjugated
MBS9460103-5x01mL	MyBiosource	5x0.1mL	EUR 2525

NKG2D Antibody FITC Conjugated
MBS9460104-01mL	MyBiosource	0.1mL	EUR 595

NKG2D Antibody FITC Conjugated
MBS9460104-5x01mL	MyBiosource	5x0.1mL	EUR 2525

NKG2D Antibody FITC-Conjugated
MBS542219-01mg	MyBiosource	0.1mg	EUR 560

NKG2D Antibody FITC-Conjugated
MBS542219-5x01mg	MyBiosource	5x0.1mg	EUR 2380

NKG2D Antibody Affinity Purified
MBS540582-01mg	MyBiosource	0.1mg	EUR 445

NKG2D Antibody Affinity Purified
MBS540582-5x01mg	MyBiosource	5x0.1mg	EUR 1855

NKG2D Antibody BIOTIN-Conjugated
MBS542218-01mg	MyBiosource	0.1mg	EUR 560

NKG2D Antibody BIOTIN-Conjugated
MBS542218-5x01mg	MyBiosource	5x0.1mg	EUR 2380

NKG2D Polyclonal Antibody
MBS9237539-01mL	MyBiosource	0.1mL	EUR 415

NKG2D Polyclonal Antibody
MBS9237539-5x01mL	MyBiosource	5x0.1mL	EUR 1841

Anti-Mouse NKG2D Antibody (CX5)
MBS1569793-01mg	MyBiosource	0.1mg	EUR 405

Anti-Mouse NKG2D Antibody (CX5)
MBS1569793-5x01mg	MyBiosource	5x0.1mg	EUR 1520

Human NKG2D Antibody (Biotin Conjugate)
36795-05121	AssayPro	150 ug	EUR 315

NKG2D Antibody Antigenic Blocking Peptide
MBS544043-025mg	MyBiosource	0.25mg	EUR 280

NKG2D Antibody Antigenic Blocking Peptide
MBS544043-5x025mg	MyBiosource	5x0.25mg	EUR 1110

Anti-Mouse NKG2D Antibody (MI-6)
MBS1569792-01mg	MyBiosource	0.1mg	EUR 405

Anti-Mouse NKG2D Antibody (MI-6)
MBS1569792-5x01mg	MyBiosource	5x0.1mg	EUR 1520

NKG2D Polyclonal Conjugated Antibody
C46825	SAB	100ul	EUR 476.4

NKG2D Polyclonal Conjugated Antibody
MBS9454723-01mLAF350	MyBiosource	0.1mL(AF350)	EUR 480

NKG2D Polyclonal Conjugated Antibody
MBS9454723-01mLAF405	MyBiosource	0.1mL(AF405)	EUR 480

NKG2D Polyclonal Conjugated Antibody
MBS9454723-01mLAF488	MyBiosource	0.1mL(AF488)	EUR 480

NKG2D Polyclonal Conjugated Antibody
MBS9454723-01mLAF555	MyBiosource	0.1mL(AF555)	EUR 480

NKG2D Polyclonal Conjugated Antibody
MBS9454723-01mLBiotin	MyBiosource	0.1mL(Biotin)	EUR 480

NKG2D Antibody Western Blot Positive Control
MBS542991-5Applications	MyBiosource	5Applications	EUR 335

NKG2D Antibody Western Blot Positive Control
MBS542991-5x5Applications	MyBiosource	5x5Applications	EUR 1350

NKG2D Ligand 2 (ULBP2) Antibody
abx005773-100g	Abbexa	100 µg	EUR 175

NKG2D Ligand 2 (ULBP2) Antibody
20-abx005773	Abbexa	Ask for price Ask for price Ask for price Ask for price	20 ul 50 ul 100 ul 200 ul

NKG2D Ligand 2 (ULBP2) Antibody
abx029272-400l	Abbexa	400 µl	EUR 518.75

NKG2D Ligand 2 (ULBP2) Antibody
abx029272-400ul	Abbexa	400 ul	EUR 627.6

NKG2D Ligand 2 (ULBP2) Antibody
abx029272-80l	Abbexa	80 µl	EUR 281.25

NKG2D Ligand 2 (ULBP2) Antibody
20-abx142298	Abbexa	Ask for price Ask for price Ask for price	50 ul 100 ul 200 ul

NKG2D Ligand 2 (ULBP2) Antibody
abx320552-100l	Abbexa	100 µl	EUR 350

NKG2D Ligand 2 (ULBP2) Antibody
20-abx320552	Abbexa	Ask for price Ask for price	50 ul 100 ul

NKG2D Ligand 2 (ULBP2) Antibody
abx320552-50l	Abbexa	50 µl	EUR 237.5

NKG2D Ligand 2 (ULBP2) Antibody
abx239250-100l	Abbexa	100 µl	Ask for price

NKG2D Ligand 2 (ULBP2) Antibody
abx239250-100ug	Abbexa	100 ug	EUR 661.2

NKG2D Ligand 2 (ULBP2) Antibody
abx239250-50l	Abbexa	50 µl	EUR 350

Human CD314 (NKG2D) Monoclonal antibody
314B-01MG	ImmunoStep	0,1 mg	EUR 218.9

Rat Monoclonal anti-Mouse CD314 (NKG2D) Antibody
xAP-0620	Angio Proteomie	100ug	EUR 280

NKG2D Polyclonal Antibody PE Conjugated
MBS9462367-01mL	MyBiosource	0.1mL	EUR 595

NKG2D Polyclonal Antibody PE Conjugated
MBS9462367-5x01mL	MyBiosource	5x0.1mL	EUR 2525

Mouse Monoclonal anti-Human CD314 (NKG2D) Antibody
xAP-0228	Angio Proteomie	100ug	EUR 280

NKG2D Polyclonal Antibody Bio Conjugated
MBS9462366-01mL	MyBiosource	0.1mL	EUR 595

NKG2D Polyclonal Antibody Bio Conjugated
MBS9462366-5x01mL	MyBiosource	5x0.1mL	EUR 2525

NKG2-D Type II Integral Membrane Protein (NKG2D) Antibody
abx430571-200l	Abbexa	200 µl	EUR 387.5

NKG2-D Type II Integral Membrane Protein (NKG2D) Antibody
abx430571-200ul	Abbexa	200 ul	EUR 460.8

NKG2D
E8ER1913-86	EnoGene	100ul	EUR 275
Description: Available in various conjugation types.

NKG2D
MBS8578073-01mL	MyBiosource	0.1mL	EUR 345

NKG2D
MBS8578073-01mLAF405L	MyBiosource	0.1mL(AF405L)	EUR 565

NKG2D
MBS8578073-01mLAF405S	MyBiosource	0.1mL(AF405S)	EUR 565

NKG2D
MBS8578073-01mLAF610	MyBiosource	0.1mL(AF610)	EUR 565

NKG2D
MBS8578073-01mLAF635	MyBiosource	0.1mL(AF635)	EUR 565

Human NKG2D AssayLite™ Antibody (RPE Conjugate)
36795-05151	AssayPro	5 x 30 ug	EUR 370

Human NKG2D AssayLite™ Antibody (APC Conjugate)
36795-05161	AssayPro	5 x 30 ug	EUR 370

Human NKG2D AssayLite™ Antibody (FITC Conjugate)
36795-05141	AssayPro	2 x 75 ug	EUR 370

Human NKG2D AssayLite™ Antibody (PerCP Conjugate)
36795-05171	AssayPro	5 x 30 ug	EUR 410

CD314 (NKG2D)
314A-100T	ImmunoStep	100 test	EUR 730.8

CD314 (NKG2D)
314F-100T	ImmunoStep	100 test	EUR 450

CD314 (NKG2D)
314PE-100T	ImmunoStep	100 test	EUR 840

CD314 (NKG2D)
314PU-01MG	ImmunoStep	0,1 mg	EUR 356.4

CD314 (NKG2D)
MO314B(V50)	ImmunoStep	50 ug	EUR 60

CD314 (NKG2D)
MO314F(V50)	ImmunoStep	50 ug	EUR 60

CD314 (NKG2D)
MO314PE(V50)	ImmunoStep	50 ug	EUR 60

Mouse Anti-Human NKG2D monoclonal antibody, clone 2E22
CABT-L1930	Creative Diagnostics	1 mg	EUR 748.13
Description: Mouse

NKG2D-IN-1
HY-155311	MedChemExpress	Get quote	Ask for price
Description: NKG2D-IN-1 (compound 21) is a natural killer group 2D receptor (NKG2D) inhibitor with IC50s of 0.3 µM and 0.7 µM in NKG2D/MICA and ULBP6 TR-FRET assays, respectively[1].

NKG2D-IN-2
HY-155312	MedChemExpress	Get quote	Ask for price
Description: NKG2D-IN-2 (compound 47) is a natural killer group 2D receptor (NKG2D) inhibitor with IC50s of 0.1 µM and 0.2 µM in NKG2D/MICA and ULBP6 TR-FRET assays, respectively[1].

NKG2D ligand 2
AP85547	SAB	1mg	EUR 2640

NKG2D Rabbit pAb
E47R0938	EnoGene	100ul	EUR 295

NKG2D Rabbit pAb
E47R21454	EnoGene	100ul	EUR 295

NKG2D Rabbit pAb
E47R41364	EnoGene	100ul	EUR 295

Armenian Hamster Anti-Mouse NKG2D Monoclonal antibody, clone HMG2D
CABT-L4409	Creative Diagnostics	1 mg; 5 mg	Ask for price
Description: Armenian Hamster

Armenian Hamster Anti-Mouse NKG2D Monoclonal antibody, clone HMG2D
CABT-L4409-1mg	Creative Diagnostics	1 mg	EUR 1076.4

Armenian Hamster Anti-Mouse NKG2D Monoclonal antibody, clone HMG2D
CABT-L4409-5mg	Creative Diagnostics	5 mg	EUR 2886

PE anti-mouse CD314 (NKG2D)
E16FMP314-050U	EnoGene	50 μg	EUR 433.33
Description: Available in various conjugation types.

PE anti-mouse CD314 (NKG2D)
E16FMP314-100U	EnoGene	100 μg	EUR 866.67
Description: Available in various conjugation types.

PE anti-mouse CD314 (NKG2D)
E16FMP314-200U	EnoGene	200 μg	EUR 1256.67
Description: Available in various conjugation types.

PE anti-human CD314 (NKG2D)
E16FHP314-025	EnoGene	25 tests	EUR 433.33
Description: Available in various conjugation types.

PE anti-human CD314 (NKG2D)
E16FHP314-100	EnoGene	100 tests	EUR 1256.67
Description: Available in various conjugation types.

Anti-Human CD314 (NKG2D) PE
MBS697097-100Tests	MyBiosource	100Tests	EUR 450

Anti-Human CD314 (NKG2D) PE
MBS697097-25Tests	MyBiosource	25Tests	EUR 250

Anti-Human CD314 (NKG2D) PE
MBS697097-5x100Tests	MyBiosource	5x100Tests	EUR 1980

Anti-Mouse CD314 (NKG2D) PE
MBS697098-005mg	MyBiosource	0.05mg	EUR 235

Anti-Mouse CD314 (NKG2D) PE
MBS697098-01mg	MyBiosource	0.1mg	EUR 310

Anti-Mouse CD314 (NKG2D) PE
MBS697098-5x01mg	MyBiosource	5x0.1mg	EUR 1355

APC anti-human CD314 (NKG2D)
E16FHA314-025	EnoGene	25 tests	EUR 541.67
Description: Available in various conjugation types.

APC anti-human CD314 (NKG2D)
E16FHA314-100	EnoGene	100 tests	EUR 975
Description: Available in various conjugation types.

APC anti-mouse CD314 (NKG2D)
E16FMA314-025U	EnoGene	25 μg	EUR 325
Description: Available in various conjugation types.

APC anti-mouse CD314 (NKG2D)
E16FMA314-050U	EnoGene	50 μg	EUR 650
Description: Available in various conjugation types.

APC anti-mouse CD314 (NKG2D)
E16FMA314-100U	EnoGene	100 μg	EUR 1126.67
Description: Available in various conjugation types.

KLRK1/CD314/NKG2D Rabbit anti-Human Polyclonal (C-Terminus) Antibody
MBS2402288-005mL	MyBiosource	0.05mL	EUR 615

KLRK1/CD314/NKG2D Rabbit anti-Human Polyclonal (C-Terminus) Antibody
MBS2402288-5x005mL	MyBiosource	5x0.05mL	EUR 2605

FITC anti-mouse (CD314) NKG2D
E16FMF314-050U	EnoGene	50 μg	EUR 390
Description: Available in various conjugation types.

FITC anti-human CD314 (NKG2D)
E16FHF314-025	EnoGene	25 tests	EUR 498.33
Description: Available in various conjugation types.

FITC anti-human CD314 (NKG2D)
E16FHF314-100	EnoGene	100 tests	EUR 1061.67
Description: Available in various conjugation types.

Biotin anti-human CD314 (NKG2D)
E16FHB314-100U	EnoGene	100 μg	EUR 780
Description: Available in various conjugation types.

Biotin anti-mouse CD314 (NKG2D)
E16FMB314-050U	EnoGene	50 μg	EUR 346.67
Description: Available in various conjugation types.

Biotin anti-mouse CD314 (NKG2D)
E16FMB314-100U	EnoGene	100 μg	EUR 693.33
Description: Available in various conjugation types.

Purified anti-mouse CD314 (NKG2D)
E16FMU314-500U	EnoGene	500 μg	EUR 1083.33
Description: Available in various conjugation types.

Purified anti-human CD314 (NKG2D)
E16FHU314-100U	EnoGene	100 μg	EUR 433.33
Description: Available in various conjugation types.

Rat Monoclonal anti-mouse NKG2D
mAP-0293	Angio Proteomie	100ug	EUR 250

Mouse Monoclonal anti-human NKG2D
hAP-0489	Angio Proteomie	100ug	EUR 250

PE-Cy7 anti-human CD314 (NKG2D)
E16FHN314-025	EnoGene	25 tests	EUR 541.67
Description: Available in various conjugation types.

PE-Cy7 anti-human CD314 (NKG2D)
E16FHN314-100	EnoGene	100 tests	EUR 1148.33
Description: Available in various conjugation types.

NKG2D / KLRK1 Immunizing Peptide
MBS426409-01mg	MyBiosource	0.1mg	EUR 225

NKG2D / CD314 Recombinant Protein
11-223	ProSci	0.1 mg	EUR 651.3
Description: NKG2D is a transmembrane protein belonging to the CD94/NKG2 family of C-type lectin-like receptors, also known as KLRK1, CD314, D12S2489E, KLR and killer cell lectin like receptor K1. NKG2D itself forms a homodimer whose ectodomains serve for ligand binding. NKG2D is a major recognition receptor for the detection and elimination of transformed and infected cells as its ligands are induced during cellular stress, either as a result of infection or genomic stress such as in cancer. In NK cells, NKG2D serves as an activating receptor, which itself is able to trigger cytotoxicity. The function of NKG2D on CD8+ T cells is to send co-stimulatory signals to activate them.

NKG2D / CD314 Recombinant Protein
11-282	ProSci	0.1 mg	EUR 651.3
Description: NKG2D is a transmembrane protein belonging to the CD94/NKG2 family of C-type lectin-like receptors, also known as KLRK1, CD314, D12S2489E, KLR and killer cell lectin like receptor K1. NKG2D itself forms a homodimer whose ectodomains serve for ligand binding. NKG2D is a major recognition receptor for the detection and elimination of transformed and infected cells as its ligands are induced during cellular stress, either as a result of infection or genomic stress such as in cancer. In NK cells, NKG2D serves as an activating receptor, which itself is able to trigger cytotoxicity. The function of NKG2D on CD8+ T cells is to send co-stimulatory signals to activate them.

NKG2D / CD314 Recombinant Protein
11-299	ProSci	0.1 mg	EUR 651.3
Description: NKG2D is a transmembrane protein belonging to the CD94/NKG2 family of C-type lectin-like receptors, also known as KLRK1, CD314, D12S2489E, KLR and killer cell lectin like receptor K1. NKG2D itself forms a homodimer whose ectodomains serve for ligand binding. NKG2D is a major recognition receptor for the detection and elimination of transformed and infected cells as its ligands are induced during cellular stress, either as a result of infection or genomic stress such as in cancer. In NK cells, NKG2D serves as an activating receptor, which itself is able to trigger cytotoxicity. The function of NKG2D on CD8+ T cells is to send co-stimulatory signals to activate them.

Human NKG2D Recombinant Protein
PROTP26718	BosterBio	Regular: 20ug	EUR 275
Description: Human NKG2D Recombinant Protein expressed in Baculovirus with hIgG-His-tag. Sequence domain: 73-216aa. Application(s): SDS-PAGE, Bioactivity. Endotoxin: < 1 EU per 1ug of protein (determined by LAL method).

NE Purified anti-mouse CD314 (NKG2D)
E16FMY314-500U	EnoGene	500 μg	EUR 1126.67
Description: Available in various conjugation types.

PerCP-Cy5.5 anti-human CD314 (NKG2D)
E16FHS314-025	EnoGene	25 tests	EUR 498.33
Description: Available in various conjugation types.

PerCP-Cy5.5 anti-human CD314 (NKG2D)
E16FHS314-100	EnoGene	100 tests	EUR 1278.33
Description: Available in various conjugation types.

NE Purified anti-human CD314 (NKG2D)
E16FHY314-050U	EnoGene	50 μg	EUR 498.33
Description: Available in various conjugation types.

NE Purified anti-human CD314 (NKG2D)
E16FHY314-500U	EnoGene	500 μg	EUR 1061.67
Description: Available in various conjugation types.

Human NKG2D / CD314, Fc Tag
E24PHA192	EnoGene	50 μg	EUR 200

UNE Purified anti-human CD314 (NKG2D)
E16FHZ314-1000U	EnoGene	1 mg	EUR 2795
Description: Available in various conjugation types.

NKG2D, Avi-Tag, Fc fusion
100252	BPS Bioscience	100 µg	EUR 320
Description: Human NKG2d, also known as, Killer Cell Lectin Like Receptor K1, KLRK1, and NK Cell Receptor D. GenBank Accession No. NM_007360.4, a.a. 38-216, with an N-terminal Avi-Tag™ fused to the Fc portion of human IgG1 and expressed in a HEK293 cell expression system. MW=53 kDa.

Human NKG2D / CD314, His Tag
E24PHA193	EnoGene	50 μg	EUR 262

Human NKG2D Protein, mFc Tag
E33P100079	EnoGene	10 μg	EUR 225

Mouse NKG2D Protein, hFc Tag
MBS189277-001mg	MyBiosource	0.01mg	EUR 260

Mouse NKG2D Protein, hFc Tag
MBS189277-005mg	MyBiosource	0.05mg	EUR 545

Mouse NKG2D Protein, hFc Tag
MBS189277-5x005mg	MyBiosource	5x0.05mg	EUR 2160

Human NKG2D Protein, mFc Tag
MBS8575114-001mg	MyBiosource	0.01mg	EUR 305

Human NKG2D Protein, mFc Tag
MBS8575114-5x001mg	MyBiosource	5x0.01mg	EUR 1220

Human NKG2D HEK293 Cell Line
E24C00248	EnoGene	each	EUR 1225
Description: Human

Mouse NKG2D HEK293 Cell Line
E24C00250	EnoGene	each	EUR 1225
Description: Mouse

Hamster monoclonal anti-mouse CD314 (NKG2D)
gAP-0033	Angio Proteomie	1mg	EUR 450

Recombinant Mouse NKG2D/CD314 Protein
RP02720	Abclonal	1000μg	EUR 219.64

Mouse CD3ζ&NKG2D EL4 Cell Line
E24C00005	EnoGene	each	EUR 1225
Description: Mouse

Human NKG2D&CD3ζ EL4 Cell Line
E24C00010	EnoGene	each	EUR 1225
Description: Human

Human NKG2D&CD3ζ EL4 Cell Line
E24C00251	EnoGene	each	EUR 1225
Description: Human

Human NKG2D&CD3ζ EL4 Cell Line
E24C00252	EnoGene	each	EUR 1225
Description: Human

Human NKG2D ligand 2 ELISA Kit
EK4447	SAB	96 tests	EUR 599

Human NKG2D ligand 2 ELISA Kit
MBS2880120-10x96StripWells	MyBiosource	10x96-Strip-Wells	EUR 4080

Human NKG2D ligand 2 ELISA Kit
MBS2880120-48StripWells	MyBiosource	48-Strip-Wells	EUR 390

Human NKG2D ligand 2 ELISA Kit
MBS2880120-5x96StripWells	MyBiosource	5x96-Strip-Wells	EUR 2220

Human NKG2D ligand 2 ELISA Kit
MBS2880120-96StripWells	MyBiosource	96-Strip-Wells	EUR 520

Human NKG2D ligand 2 ELISA Kit
MBS761361-10x96StripWells	MyBiosource	10x96-Strip-Wells	EUR 3900

Human NKG2D ligand 2 ELISA Kit
MBS761361-48StripWells	MyBiosource	48-Strip-Wells	EUR 340

Human NKG2D ligand 2 ELISA Kit
MBS761361-5x96StripWells	MyBiosource	5x96-Strip-Wells	EUR 2045

Human NKG2D ligand 2 ELISA Kit
MBS761361-96StripWells	MyBiosource	96-Strip-Wells	EUR 455

Human NKG2D ligand 2 ELISA Kit
MBS9428273-5x96Tests	MyBiosource	5x96Tests	EUR 2820

Results: A prominent raise of miR-218 was observed in DKD through bioinformatics analysis, which was further confirmed in the HG-induced model. DACH1 is a target of miR-218. miR-218 reduced cell viability and induced apoptosis by negatively regulating DACH1. Moreover, upregulating miR-218 in HG models increased the concentrations of pro-inflammatory cytokines TNF-α and IL-1β, reduced the level of anti-inflammatory cytokine IL-10, and promoted the epithelial-mesenchymal transition (EMT) process, which is possibly achieved by targeting DACH1.

While downregulating miR-218 showed the opposite results.Conclusion: These data demonstrated that, under an in vitro HG environment, miR-218 suppressed the HK-2 cells proliferation, promoted apoptosis, caused an inflammatory response, and facilitated the EMT process largely by targeting DACH1, providing an insight into the therapeutic intervention of DKD.